What is it about?
The way qPCR experiments are handled is extremely important for the outcome of your research. Using the wrong normalisation method for instance, can make a difference between significant and non-significant results, but also between nonsenses results and sound evidence. This paper handles a robust way to normalise miRNA qPCR experiments. Since it makes use of all the published evidence on the internet, the method to create a normalisation panel is very sound. This is confirmed by the excellent precision of the normalisation panel.
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Perspectives
I would like to stress the importance of this paper, since most of the time people are only interested in the outcome of their experiment, but do not think too much about the study design. The study design on the other hand can make or break your experiment and is in great part responsible for the inconsistencies published on similar subjects. This study shows, that this is the most robust way to normalise miRNA PCR experiments. I believe especially the precision of this methods is clearly shown. If somebody knows a better way to normalise I would really like to get in contact, to be able to improve this so important aspect of conducting a good study.
Dr Sara-Joan Pinto-Sietsma
Universiteit van Amsterdam
Read the Original
This page is a summary of: Normalization panels for the reliable quantification of circulating microRNAs by RT-qPCR, The FASEB Journal, May 2015, Federation of American Societies For Experimental Biology (FASEB),
DOI: 10.1096/fj.15-271312.
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