What is it about?
Our study aimed to develop tools that target specific regions in our DNA, and simultaneously insert a foreign DNA fragment in those locations. The foreign DNA sequence can alter gene function, either by helping us better control normal genes or through halting and blocking rogue genes. In our research, we have combined two different protein molecules that have enzymatic activity. One molecule helps find specific DNA sequences and the other helps to insert new DNA sequences in the correct gene location.
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Photo by Zoltan Tasi on Unsplash
Why is it important?
We found that the designed fused molecule functioned as we expected and was able to specifically insert DNA sequences in the desired location. This method could potentially be very useful in targeting and blocking genes that cause different diseases, especially in end-stage cancer.
Perspectives
I hope the findings of this paper could be useful for gene targeting applications.
Dr. Hesam Dehghani
Ferdowsi University of Mashhad
Read the Original
This page is a summary of: CRISPR/dCas9‐mediated transposition with specificity and efficiency of site‐directed genomic insertions, The FASEB Journal, January 2021, Wiley,
DOI: 10.1096/fj.202001830rr.
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