β-Actin–dependent global chromatin organization and gene expression programs control cellular identity

Xin Xie, Bader Almuzzaini, Nizar Drou, Stephan Kremb, Ayman Yousif, Ann-Kristin Östlund Farrants, Kristin Gunsalus, Piergiorgio Percipalle
  • The FASEB Journal, March 2018, Federation of American Societies For Experimental Biology (FASEB)
  • DOI: 10.1096/fj.201700753r

Beta-actin impact on the nuclear genome organization, transcription programs.

What is it about?

Using beta-actin knockout Mouse Embryonic Fibroblasts, we showed the changes in the heterochromatin organization, which is associated with dramatic changes in genes involved in common cellular processes. Those gene programs changes lead to differential phenotypical cellular identity.

Why is it important?

This study highlighted the importance of beta-actin especially in the nucleus to influence the chromatin organization and gene programs. This implies an essential function of beta-actin in the establishment of cell fate and identity.

Perspectives

Xin Xie (Author)

Our study showed the loss of chromatin binding of BAF complex subunit Brg1with beta-actin as a partner. Further work needs to clarify at genomic level how other chromatin remodeling complexes such as Polycomb complex are affected without beta-actin in the nucleus.

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http://dx.doi.org/10.1096/fj.201700753r

The following have contributed to this page: Dr Piergiorgio Percipalle and Xin Xie