What is it about?
A new cloning method to produce seamless/scarless insertions. A method to transfer large pieces of genomic DNA between large plasmids (Bacterial artificial chromosomes, BACs).
Why is it important?
Cloning large fragments of DNA, like entire genomic genes, usually involve multistep cloning, and hardly to join such pieces seamlessly. Our method improves on the previous methods and allows complex DNA recombinations in 2 steps.
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This page is a summary of: Assisted large fragment insertion by Red/ET-recombination (ALFIRE)—an alternative and enhanced method for large fragment recombineering, Nucleic Acids Research, May 2007, Oxford University Press (OUP), DOI: 10.1093/nar/gkm250.
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