What is it about?

A new cloning method to produce seamless/scarless insertions. A method to transfer large pieces of genomic DNA between large plasmids (Bacterial artificial chromosomes, BACs).

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Why is it important?

Cloning large fragments of DNA, like entire genomic genes, usually involve multistep cloning, and hardly to join such pieces seamlessly. Our method improves on the previous methods and allows complex DNA recombinations in 2 steps.

Perspectives

The understanding of the genome structure and functions require the use of large genetic loci to understand their functions, complex interactions between regions far away, enhancers, repressors, etc. This will allow better understanding of genetic networks in health and disease conditions.

Professor Adolfo Rivero-Muller
Uniwersytet Medyczny w Lublinie

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This page is a summary of: Assisted large fragment insertion by Red/ET-recombination (ALFIRE)—an alternative and enhanced method for large fragment recombineering, Nucleic Acids Research, May 2007, Oxford University Press (OUP), DOI: 10.1093/nar/gkm250.
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