What is it about?
ABSTRACT: The mitotic separase cleaves Scc1 in cohesin to allow sister chromatids to separate from each other upon anaphase onset. Separase is also required for DNA damage repair. Here, we isolated and characterized 10 temperature-sensitive (ts) mutants of separase ESP1 in the budding yeast Saccharomyces cerevisiae. All mutants were defective in sister chromatid separation at the restricted temperature. Some esp1-ts mutants were hypersensitive to the microtubule poison benomyl and/or the DNA-damaging agent bleomycin. Overexpression of securin alleviated the growth defect in some esp1-ts mutants, whereas it rather exacerbated it in others. The Drosophila Pumilio homolog MPT5 was isolated as a high-dosage suppressor of esp1-ts cells. We discuss various features of separase based on these findings.
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Why is it important?
Overexpression of securin alleviated the growth defect in some esp1-ts mutants, whereas it rather exacerbated it in others.
Perspectives
We discuss various features of separase based on these findings.
Dr Muhammad Waliullah Talukdar
Shizuoka University
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This page is a summary of: Elucidation of novel budding yeast separase mutants, Bioscience Biotechnology and Biochemistry, November 2015, Taylor & Francis,
DOI: 10.1080/09168451.2015.1101337.
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