Evaluation and Exploration of Lactic Acid Bacteria for Preservation and Extending the Shelf Life of Fruit

Vishal Dhundale, Vijayshree Hemke, Dhananjay Desai, Pawan Dhundale
  • International Journal of Fruit Science, February 2018, Taylor & Francis
  • DOI: 10.1080/15538362.2018.1435331

Evaluation and Exploration of LAB for Preservation and Extending the Shelf Life of Fruit

Why is it important?

The bio-preservation of fruits using bacteriocinogenic lactic acid bacteria (BLAB) isolated directly from cow dung and curd was an innovative approach. The objectives of this study were to isolate and identify Bacteriocin producing LAB from various curd and cow dung samples, evaluate their antimicrobial effects of BLAB against selected fruits spoiling and pathogenic microorganisms in vitro. LAB was isolated using MRS media. The well-diffusion bioassay was used to screen for Bacteriocin-Like Inhibitory Substance (BLIS) producing LAB Salimicrobium Bacillus, Paenibacillus and Lactobacillus. BLIS producing LAB were tested against 25 fruit flora of selected fruits. These LABs were also tested against Escherichia coli, Staphylococcus aureus, Bacillus cereus, Pseudomonas aeroginosa, Proteus vulgaris, Salmonella typhi, Serritia spp, Xanthomonas campestris, and also against Aspergillus flavus, Aspergillus nigar, Fusarium, Alternaria, Saccharomyces cerevisiae using the diffusion bioassay plate method. The antagonistic activity was also studied against pathogenic fruit flora which has been isolated from fruits. These BLISs showed a wide range of antibacterial activity against tested, fruit-spoilage and fruit-borne pathogens and able to extend the shelf life of fruits. Bacteriocinogenic LAB present in curd and cow dung may have potential to be used as bio-preservatives of tested fruits.


Dhananjay Desai (Author)
ICAR-Central Coastal Agricultural Research Institute Goa

In the present studies, total 25 fruit flora bacterial cultures were isolated from seven fruits and nine Bacteriocinogenic LAB isolated from curd and cow dung sample. The isolated Bacteriocinogenic LAB was found antibacterial activity against fruit flora as well as pathogenic microorganism and revealed best agents for fruit preservative and extends the shelf life of fruit. The BLIBs were applicable for the biological preservative towards the fruits as well as these secondary role of probiotics are very advantageous for the health as maintenance of normal microbial flora of intestine and prevention of side effect of antibiotics.

Read Publication


The following have contributed to this page: Dhananjay Desai