What is it about?
1. We developed a single-cell polyadenylation sequencing approach and used it to decipher the dynamics of polyA site usage at single-cell resolution. 2. We found many genes using multiple polyA sites in bulk data are prone to use only one polyA site in each single cell. 3. Cell cycle genes were significantly enriched in genes with high variation in polyA site usages. These results indicate that polyA site usage is an important layer for cell cycle regulation.
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Why is it important?
1. The single-cell polyadenylation sequencing developed by us could decipher the dynamics of polyA site usage at single-cell resolution. 2. We found the genes showing polyA site usage changes during cell cycle were grouped into six clusters, with cell phase-specific functional categories enriched in each cluster. These results indicate that polyA site usage is an important layer for cell cycle regulation.
Perspectives
We expect more genes showing polyA usage switch during cell cycle will be identified. Furthermore, the mechanism underlying polyA site usage switch shaping cell cycle will be identified in the future.
Prof. Wenfei Jin
Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences
Read the Original
This page is a summary of: Comprehensive mapping of alternative polyadenylation site usage and its dynamics at single-cell resolution, Proceedings of the National Academy of Sciences, December 2022, Proceedings of the National Academy of Sciences,
DOI: 10.1073/pnas.2113504119.
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