What is it about?
Neutrophils and NETs have been described to play an important role in thrombosis. We have shown that neutrophils are involved in arterial thrombosis. Here, we used Serial Block Face electron microscopy to discriminate the presence of Neutrophils and NETs into a thrombus. We also studied the effect of DNASE-I on platelet and neutrophil activation. Last, we determined if commonly used NETs markers are not also markers of neutrophil activation.
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Photo by Landon Arnold on Unsplash
Why is it important?
Our findings show that neutrophils but not NETs are important in one model of immuno-thrombosis. CitH3, NE and PAD4 are markers of neutrophil activation. We show that DNAse-I directly affects platelet aggregation and neutrophil activation by hydrolyzing ATP and ADP in Adenosine. The presence and role of NETs need to be re-evaluated in other models.
Perspectives
We were really proud to obtain 3-dimensional images of a thrombus at this level of definition (do not miss the movie!). We were also really surprised to observe that DNAse-I affects thrombosis by degrading ATP and ADP. More than anything else we hope you find this article thought-provoking!
Christophe Dubois
Aix-Marseille Universite
Read the Original
This page is a summary of: DNAse-dependent, NET-independent pathway of thrombus formation in vivo, Proceedings of the National Academy of Sciences, July 2021, Proceedings of the National Academy of Sciences,
DOI: 10.1073/pnas.2100561118.
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