Study on Alternaria alternate, Fusarium chlamydosporum accompanying the Eucalyptus camaldulensis L. trees in some regions of Kerbala province/Iraq and identification of isolate with PCR technique

What is it about?

The Eucalyptus genus belongs to the family of the America Myrtaceae, order of Wallace and Ocimum, And it is a tree evergreen a large size may reach a height of 100 m, It has 100 species of 3500 species living in temperate and temperate regions of the world, a large percentage of them are of great economic importance because their timber is involved in the manufacture of paper pulp, cellulose preparation, wood making, coal, wood pressing (Nahal, 2003), While its secondary products were the extraction of tannins from their dung and tanning as well as the extraction of volatile oils from the oil glands of leaves and contain on ketone, which are used for medical purposes, such as treatment of respiratory infection and the middle ear and inhibit the growth of bacteria

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Why is it important?

The experiment was carried out in the plastic canopy belonging to the colloge of Agriculture/ Kerbala University seedlings free from fungal prepared and insect infections (intact) at the age of 18 months obtained from one of the nurseries in Kerbala governorate , Three branches were selected from each seedling and one was injured by a 4 mm diameter flannel hole in the ditch area. The ditch was removed with a sterile knife length of 2 cm and at a height of 5 cm from the surface of the soil and vaccinated with a disk of developing fungi on the PDA medium 4 mm (Lewis Jr and Van Arsdel, 1978), The wound covered after vaccination with a sterile wet cotton stopper and the disc connected with a parafilm wax bar to prevent contamination with pathogens[18]Each treatment included three replicates, The results were calculated by calculating the mean increase in the length of canker that occurred two months after vaccination. Then results statistically analyzed.

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