What is it about?
Chemically modified mesoporous silica nanoparticles (MSNs) are of interest due to their chemical and thermal stability with adjustable morphology and porosity, therefore, it was aimed to develop and compare the MCM-41 MSNs functionalized with imidazole groups (MCM-41-Im) to unmodified (MCM-41-OH) and primary amine functionalized (MCM-41-NH2) MSNs for experimental gene delivery. The results show efficient transfection of the complexes of the plasmid and either MCM-41-NH2 or MCM-41-Im. Furthermore, following transfection of HeLa cells using MCM-41-Im, an enhanced GFP expression was achieved consistent with the noticeable DNase1 protection and endosomal escape properties of MCM-41-Im using carboxyfluorescein tracer.
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Why is it important?
Imidazole modification of MCM-41 synthesis was a novel method for facilitating gene condensation and delivery via mesoporous silica nano-particles. Low sytotoxity and high capacity of over coming cellular barriers made this product suitable vehicle for gene delivery.
Perspectives
Improvement of gene delivery methods is of the most important steps for gene therapy propose. using MSNs because of its bio-compatibility and chemical characteristics met many of needs in this process and I think it could be a safe way of gene delivery for specifics tissues.
mahdokht Mahmoodi
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This page is a summary of: Co-condensation synthesis of well-defined mesoporous silica nanoparticles: effect of surface chemical modification on plasmid DNA condensation and transfection , IET Nanobiotechnology, December 2017, the Institution of Engineering and Technology (the IET),
DOI: 10.1049/iet-nbt.2017.0078.
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