What is it about?

A highly sensitive and rapid HBV screening method combining membrane protein purification with surface-enhanced Raman spectroscopy was developed in this paper. Cellulose acetate (CA) membrane was utilized to extract and purify total protein from serum, then Ag colloids was applied on the menbrane to enhance the Raman signal of protein, the different expression of protein signal between normal and HBV patients could be further used for early screening of HBV.

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Why is it important?

Serum is a complex bio-matrix and the content of biochemical composition may effect by many endogenous biomolecules secreted from lesion tissue or the interference of exogenous substances from food and medicine. A serum sample pretreatment method were introduced to reduce external interference by membrane protein purification, which can be acted as a highly-sensitive and reliable indicator for disease diagnosis.


Hepatitis B virus (HBV) infection remains a significant worldwide public health problem especially in developing countries, in where indicates that a rapid, highly sensitive and cost-effective screening method for HBV is urgently needed. We hope our facile method could be an reliably supplymentary or alternative for current golden standard.

Tingyin Wang
Fujian Normal University

Read the Original

This page is a summary of: A serum analysis method combining membrane protein purification with surface-enhanced Raman spectroscopy for noninvasive HBV screening, IET Nanobiotechnology, November 2019, the Institution of Engineering and Technology (the IET),
DOI: 10.1049/iet-nbt.2019.0274.
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