Why is it important?
With the first solved structure for GluDH could we now engineer new specificity? Recognition of the substrate's carboxylate sidechain depended on Lys-89 and Ser-380. By changing these to Leu and Ala and also mutating Ala 163 to Gly we eliminate Glu activity and made a dehydrogenase with new specificity for Met and analogous norleucine.
The following have contributed to this page: Professor Paul C Engel
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