What is it about?

We have applied three techniques (atomic force microscopy, gel assays and surface plasmon resonance) to study the subunit assembly of an enzyme called EcoR124I, known as a Type IC Restriction–Modification enzyme.

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Why is it important?

The paper shows that this multi-subunit enzyme is an ideal model system for studying protein-protein interactions.


This paper also explains how to retrieve molecular volume information of proteins from AFM images and compare such data to theoretical volumes derived from molecular mass.

Dr James R Smith
University of Portsmouth

Read the Original

This page is a summary of: Cofactor induced dissociation of the multifunctional multisubunit EcoR124I investigated using electromobility shift assays, AFM and SPR, RSC Advances, January 2017, Royal Society of Chemistry,
DOI: 10.1039/c7ra07505g.
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