Investigation of eszopiclone water-induced degradation kinetics by HPTLC

What is it about?

An eco-friendly, sensitive and selective high-performance thin-layer chromatographic method was developed and validated for analysis of eszopiclone in bulk powder and formulation. The study of the eszopiclone water-induced degradation kinetics was carried out by the proposed HPTLC method. The activation energy and degradation rate constant at room temperature were calculated after the construction of the Arrhenius plot

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Why is it important?

Introducing HPTLC in pharmaceutical analysis represents a major step in terms of quality assurance. The developed HPTLC technique is eco-friendly, precise and accurate. Statistical analysis proves that the method is suitable for the analysis of eszopiclone as a bulk drug and in pharmaceutical formulations without any interference from the excipients. Comparing with previously published papers using HPLC or UHPLC for eszopiclone determination, the proposed HPTLC method has its advantages of low operating costs, using an eco-friendly mobile phase, and the need for minimum sample preparation. The major advantage of HPTLC is that several samples can be run simultaneously using a small quantity of the mobile phase. The proposed HPTLC method has proved to be more selective and specific than the previously published spectrophotometric method. The proposed HPTLC method is better than the previously described UPLC method for separation of eszopiclone impurities as it studied the degradation kinetics of eszopiclone without using organic solvents that may cause atmospheric contamination. Also the degradation rate constant, half-life and t90 of eszopiclone can be predicted.

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