What is it about?
There is nolonger a need for purifying your enzyme and testing various immobilization techniques, before employing it as catalyst in your reactions. EziG immobilizes any His-tagged enzyme, and purifies it, in a simple step, on an inert porous material which is suitable for any solvent. The method greatly facilitates biocatalytic process development.
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Why is it important?
Biocatalysis is a growing field and a key to a sustainable chemical industry. However, there is a lack of general methods for reaching a suitable biocatalyst for a given transformation. This work demonstrates how several steps in the process of purification and immobilization can be reduced to one straight-forward technique, which is generally applicable.
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This page is a summary of: A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications, Chemical Communications, July 2014, Royal Society of Chemistry,
DOI: 10.1039/c4cc02605e.
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