Separation and quantification of cysteine, glutathione and phytochelatins in rice (Oryza sativa L.) upon cadmium exposure using reverse phase ultra performance liquid chromatography (RP-UPLC) with fluorescence detection

  • Zeying Wu, Chunhua Zhang, Jiali Yan, Ying Ge
  • Analytical Methods, January 2013, Royal Society of Chemistry
  • DOI: 10.1039/c3ay40849c

Quantification of thiol compounds in rice

What is it about?

A simple, fast and reliable technique was established to analyze thiol compounds in different parts of rice samples.

Why is it important?

Thiols are low level and easy to be oxidized, making their quantification difficult to achieve. Using sensitivity and resolution of UPLC, we were able to separate and determine each thiol molecule in a short time (around 30 min per sample). This technique will save both time and reagent in the analysis of thiols.

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