What is it about?

Herein we demonstrate the first example of oligonucleotide templated reaction (OTR) performed on paper, using lateral flow assays for amplification-free detection of circulating miRNA (miR-150-5p), a recently identified predictive blood biomarker for preterm birth.

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Why is it important?

The majority of preterm births (PTBs) are to women with no identifiable risk factors, making cases extremely hard to predict at an early stage, when the mother would benefit the most from medical treatment. Low-cost, paper-based blood tests for early prediction of PTB could provide a valuable alternative to current approaches that could improve neonatal outcomes.


This investigation marks a step in our effort to develop a point of care test for early detection of preterm birth. One of the findings pertaining to PNA mobility on the LFA sample pad is an important one. The key observation made is that unhybridized PNA does not travel through the cellulose sample pad unless hybridized to a complementary DNA or RNA. This could have implications in further iterations of LFA devices.

Suraj Pavagada
University of Cambridge

Read the Original

This page is a summary of: Oligonucleotide-templated lateral flow assays for amplification-free sensing of circulating microRNAs, Chemical Communications, January 2019, Royal Society of Chemistry, DOI: 10.1039/c9cc05607f.
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