What is it about?

Electron-phonon coupling in monolayer graphene results in a modification of its Raman spectra upon charge transfer processes induced by interaction with its chemical environment or the presence of strain or defects in its structure. Modification of Raman spectra is examined in order to develop ultra-sensitive biosensing techniques for the detection, identification, differentiation and classification of bacteria connected with infectious diseases. Specifically, the electrochemical properties of top gated monolayer graphene on SiO2/Si substrates, in the absence and presence of interaction with Gram-positive bacteria (Enterococcus faecalis, Bacillus subtilis) and Gram-negative bacteria (Escherichia coli and Salmonella typhimurium), are probed by Raman spectroscopy in an applied voltage range from 0 V to 3 V.

Featured Image

Why is it important?

Bacteria and monolayer graphene interactions are thus electrostatically tuned. The resulting correlation of specific bacterial chemical properties and Raman spectral characteristics is reported, along with density functional theory simulations of the charge transfer mechanism. The intensities of the G and D Raman vibrational modes are modulated as a function of applied voltage in the presence of bacteria, but remain unchanged in bare monolayer graphene.


A fingerprint region is also identified in the range of 200 cm-1 to 600 cm-1, with disulfide bonds observed at 490 cm-1, associated with bacterial membrane proteins. Significantly, such observations are detected even in the absence of bacterial culturing, a time-consuming step.

Dr. Sitansu Sekhar Nanda
Myongji University

Read the Original

This page is a summary of: A new device concept for bacterial sensing by Raman spectroscopy and voltage-gated monolayer graphene, Nanoscale, January 2019, Royal Society of Chemistry,
DOI: 10.1039/c8nr10010a.
You can read the full text:



The following have contributed to this page