What is it about?
In human mesenchymal stem cells (hMSCs), sorting out a target subpopulation from the others is very important to increase the effectiveness of cell-based therapy. In this paper, we performed flow-based sorting of hMSCs by using optimally designed microfluidic-chips based on the hydrodynamic filtration (HDF) principle. The microfluidic-chip has main, side, and multiple branch channels, where each channel is divided and connected to three outlets for trimodal separation of hMSCs as small, medium, and large groups.
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Why is it important?
Our microfluidic-chip was designed with the parameters rigorously determined by the complete analysis of laminar flow and complicated networks of main and multi-branched channels for hMSCs sorting. This opens the possibility of sorting stem cells rapidly with high multipotency without damage. Over 86% recovery was achieved for each population of cells with complete purity in small cells.
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This page is a summary of: Sorting of human mesenchymal stem cells by applying optimally designed microfluidic chip filtration, The Analyst, January 2015, Royal Society of Chemistry, DOI: 10.1039/c4an01430h.
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