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Describes construction of the first shuttle plasmids able to be propagated in both bacterial and eukaryotic cells. These are based on the 2 micron plasmid of Saccharomyces cerevisiae that is able to replicate up to high copy number in yeast cells.These plasmids are used to demonstrate high efficiency yeast transformation followed by recovery of the plasmids in E. coli. It is also shown that the 2 micron plasmid undergoes recombination across its inverted repeat sequences when propagated in yeast.
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This page is a summary of: Transformation of yeast by a replicating hybrid plasmid, Nature, September 1978, Springer Science + Business Media,
DOI: 10.1038/275104a0.
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