Trade-offs in Engineering Sugar Utilization Pathways for Titratable Control

  • Taliman Afroz, Konstantinos Biliouris, Kelsey E. Boykin, Yiannis Kaznessis, Chase L. Beisel
  • ACS Synthetic Biology, February 2015, American Chemical Society (ACS)
  • DOI: 10.1021/sb400162z

Using native sugar utilization pathways as inducible systems in microorganisms

What is it about?

Inducible systems are important tools to study and engineer microorganisms. Sugar utilization offers readily available inducible systems, yet they often exhibit undesirable traits. Here, we explore how to modify these pathways to improve these traits, such as strengthening induction or ensuring all cells in the population behave similarly. We found that every modification came with trade-offs, improving one trait while hurting another.

Why is it important?

Non-model microorganisms are increasingly being explored as genetic systems or for industrial applications. Our findings guide how to manipulate native sugar utilization pathways in order to drive our understanding and control of these microorganisms.

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http://dx.doi.org/10.1021/sb400162z

The following have contributed to this page: Chase Beisel and Yiannis Kaznessis