Indirect covariance mathematical processing to create an equivalent GHSQC-TOCSY experiment

What is it about?

GHSQC-TOCSY is a relatively insensitive 2D NMR experiment. Given two coherence transfer experiments, A → B and A → C, it is possible to indirectly determine B ↔ C. Unsymmetrical indirect covariance processing of a 1H–13C GHSQC and a GCOSY spectrum afforded a GHSQC-COSY spectrum, with an information content analogous to a GHSQC-TOCSY experiment.

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Why is it important?

GHSQC-TOCSY experiments allow sorting of proton–proton connectivity information as a function of 13C chemical shift. Investigators needing access to GHSQC-TOCSY type data can, access it from more readily acquired 2D NMR data, specifically 1H–13C GHSQC and GCOSY spectra.