What is it about?
We have continued the development of our mass spectrometry platform which uses acoustic tone bursts to generate a mist spray for sample analysis. This paper presents modifications made to the original prototype and demonstrates the application of this technology for biochemical high-throughput screening. In the paper we share data from a 2M compound collection screen to find inhibitors of a disease relevant target. Using acoustics and simple automation it is possible to generate 100,000 data points per day from a single mass spectrometer. Simple automation was added to enable this level of throughput and we have developed data analysis tools capable of supporting this unprecedented speed of data acquisition.
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Why is it important?
This is the first example of an electrospray like mass spectrometry platform with the ability to support a full HTS campaign of a library in excess of 1 million samples. The throughput of 100,000 samples per day is truly revolutionary for high throughput MS analysis.
Perspectives
There has been growing interest in high throughput mass spectrometry in recent years. This paper demonstrates the potential to use acoustics to interface with MS detectors to significantly increase the sample loading rates. This paper builds on our original work and demonstrates that even with a prototype it is possible to robustly screen significant numbers of compounds. We have used this opportunity to build a complete solution including automation and data analysis ahead of commercialization. We are now looking for other applications for this technology.
Dr jonathan wingfield
AstraZeneca plc
Read the Original
This page is a summary of: Acoustic Mist Ionization Platform for Direct and Contactless Ultrahigh-Throughput Mass Spectrometry Analysis of Liquid Samples, Analytical Chemistry, March 2019, American Chemical Society (ACS),
DOI: 10.1021/acs.analchem.9b00142.
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