What is it about?
ADP-ribosylation is a difficult to study post-translational modification (PTM), with numerous biological roles. We have developed methods that enable the simple incorporation of ADP-ribose into peptides and proteins for deconvoluting the roles played by this PTM.
Why is it important?
ADP-ribosylation is an important PTM, associated with DNA damage repair and cell death. It exists as a monomer, and linear or branched polymers, that can be attached through the side chain of various amino acids via chemical or enzymatic means. Due to the metabolic and chemical instability of this modification, the capacity to purify large amounts of site-specifically modified ADP-ribosylated peptides and proteins to study its role is limited. This paper provides a simple means to generate such molecules, which may provide a useful tool for studying this PTM. We also demonstrate the utility of photocrosslinking as a tool to improve our capacity to identify proteins that bind this modification.
The following have contributed to this page: Dr Peter Michael Moyle