Site-Specific Incorporation of Three Toll-Like Receptor 2 Targeting Adjuvants into Semisynthetic, Molecularly Defined Nanoparticles: Application to Group A Streptococcal Vaccines

Peter M. Moyle, Wei Dai, Yingkai Zhang, Michael R. Batzloff, Michael F. Good, Istvan Toth
  • Bioconjugate Chemistry, May 2014, American Chemical Society (ACS)
  • DOI: 10.1021/bc500108b

Efficient method to incorporate TLR2 ligands into recombinant protein antigens

What is it about?

This paper provides an efficient means to site-specifically incorporate potent immunostimulatory TLR2 ligands (e.g. Pam2Cys, Pam3Cys and LCP) site-specifically onto recombinant protein antigens. We also demonstrate the utility of this system when combined with engineered polytopic recombinant proteins for eliciting antibodies capable of preventing infection with group A streptococcus, an important disease associated bacterium.

Why is it important?

Many protein antigens have been identified in the literature, however potent immunostimulatory adjuvants are needed for the successful use of these antigens in commercial vaccines. The conjugation of adjuvants to antigens ensures that all vaccine components reach the same cell, improving vaccine potency. Our technology also ensures that we produce a commercially viable, molecularly-defined vaccine, rather than a mixture of compounds.

Perspectives

Dr Peter Michael Moyle (Author)
University of Queensland

This is an example of our laboratories core technology platform, allowing for the site-specific conjugation of various types of adjuvants onto recombinant protein antigens. This technology is applicable to vaccines targeting many different diseases, and ensures that we produce a defined vaccine.

The following have contributed to this page: Dr Peter Michael Moyle and Miss Wei WD Dai