What is it about?
The aim of this study was to describe the development of zebrafish embryos that underwent cooling until reaching the adult stage, analyzing survival, growth and macro and microscopic damage. The embryos selected with 50% epiboly stage were submerged in cryoprotectant solution of methanol and sucrose and cooled gradually to a temperature of 0 ± 2° C, when they were divided into two storage times (6 and 18 hours).
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Why is it important?
This study is important because the monitor the development of Danio rerio embryos, submitted to cooling, until reaching full length for mature sexual fish, analyzing the hatching, growth and possible structural and ultrastructural damages caused by this process, will improve existing cryopreservation protocols, alleviate problems and provide basic knowledge for future studies.
Perspectives
This study is important because the monitor the development of Danio rerio embryos, submitted to cooling, until reaching full length for mature sexual fish, analyzing the hatching, growth and possible structural and ultrastructural damages caused by this process, will improve existing cryopreservation protocols, alleviate problems and provide basic knowledge for future studies.
Maria do Carmo Paes
Universidade Estadual Paulista Julio de Mesquita Filho
Read the Original
This page is a summary of: Post-cooling survival, growth and deformity rates in zebrafish embryos (Danio rerio), Zygote, December 2017, Cambridge University Press,
DOI: 10.1017/s0967199417000715.
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