What is it about?
We analysed the potential use of 17 candidate reference genes across a diverse set of samples, including several tissues, different stages and environmental conditions, encompassing seed germination and seedling growth in Ricinus communis L. These genes were tested by RT-qPCR and ranked according to the stability of their expression using two different approaches: GeNorm and NormFinder.
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Why is it important?
The approach used in this study to identify stably expressed genes, and thus potential reference genes, was applied successfully for R. communis and it provides important guidelines for RT-qPCR studies in seeds and seedlings for other species (especially in those cases where extensive microarray data are not available).
Read the Original
This page is a summary of: Identification of reference genes for gene expression studies during seed germination and seedling establishment in Ricinus communis L., Seed Science Research, September 2014, Cambridge University Press,
DOI: 10.1017/s0960258514000294.
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