What is it about?
We provide a description of the development and validation of a single-serum-dilution based enzyme-linked immunosorbent assay (ELISA) kit with ready-to-use reagents for measuring antifilarial IgG4 antibodies. Detection was performed by naked-eye observation of the resultant colour of the immunological reactivity.
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Why is it important?
This paper gave detail about process of an ELISA test kit development. Thus, this process can be a model for other researcher who is interested to develop any ELISA based test kit.
Perspectives
The author has more than 15 years experience in immunological and molecular biology researches on filariasis
sirichit wongkamchai
Mahidol University
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This page is a summary of: An ELISA kit with two detection modes for the diagnosis of lymphatic filariasis, Journal of Helminthology, May 2014, Cambridge University Press,
DOI: 10.1017/s0022149x14000522.
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