What is it about?
RNA interference (RNAi) is emerging as an alternative pest control technology. However, currently, this technology is still in the infancy stage for controlling insect pests. The findings described in this paper provides information on improving the RNAi effect for management of insect pests. RNAi effect can be enhanced by multiple times delivery of dsRNA, thus aid in efficient knockdown of gene function, eventually lead to pest management.
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Why is it important?
We optimized the method for efficient and sustained knockdown of target gene function. This is achieved by delivering the double-stranded RNA at multiple times. This results in efficient, cumulative and sustained silencing of target gene function. This is essential in the case of Lepidopteran insects where RNAi was found to be less efficient.
Perspectives
Using this approach, we can manage the agricultural pests at the field, by delivering the double-stranded RNA (dsRNA) multiple times either by spraying or by continuously expressing dsRNA in the host plants.
Sharath Chandra
Read the Original
This page is a summary of: Effect of diet delivered various concentrations of double-stranded RNA in silencing a midgut and a non-midgut gene of Helicoverpa armigera, Bulletin of Entomological Research, April 2013, Cambridge University Press,
DOI: 10.1017/s0007485313000138.
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