What is it about?
The chains of Paramecium cells and different kinds of the monsters have been obtained in axenic media of diverse composition. In cultures fed in excess (in double concentrated standard axenic medium) such forms appeared 3–4 days after inoculation.Fluorescent labelling of living cell surface with CDC (cycloheptaamylose dansyl chloride complex, Polysciences, USA) and afterwards of nuclear apparatus with DAPI has been applied for characterization of the obtained forms. CDC labelling demonstrated the continuity of the surface membrane along the whole monsters and revealed a division furrow which had not been completed in the majority of chains formed. Dislocation of macro- and micronuclei as well as intercalary division has been detected in examined abnormal forms.
Featured Image
Why is it important?
The observed monstrosity has not been inherited: transferring to normal medium gave the normal specimens which in turn formed monsters in overfed conditions.
Perspectives
The described abnormal forms of P. aurelia seem to be an interesting model in cell biology research and the technique of double sequential fluorescent labelling has been found especially useful in these studies.
Professor Elzbieta Wyroba
Nencki Institute of Experimental Biology
Read the Original
This page is a summary of: Induced Formation of Abnormal Cells in Axenic Culture of Paramecium aurelia, Archiv für Protistenkunde, January 1984, Elsevier,
DOI: 10.1016/s0003-9365(84)80029-9.
You can read the full text:
Contributors
The following have contributed to this page
