Mechanical-chemical analyses and sub-chronic systemic toxicity of chemical treated organic bovine bone

Kwang-il Lee, Jung-soo Lee, Keun-soo Lee, Hong-hee Jung, Chan-min Ahn, Young-sik Kim, Young-bock Shim, Ju-woong Jang
  • Regulatory Toxicology and Pharmacology, December 2015, Elsevier
  • DOI: 10.1016/j.yrtph.2015.10.027

Toxicity study of chemical treated bovine xenograft

What is it about?

Mechanical-chemical analyses and sub-chronic systemic toxicity of chemical treated organic bovine bone

Why is it important?

Sequentially chemical-treated bovine bone was not only evaluated by mechanical and chemical analyses but also implanted into the gluteal muscles of rats for 12 weeks to investigate potential local pathological effects and systemic toxicities. The test (chemical treated bone) and control (heat treated bone) materials were compared using scanning electron microscope (SEM), x-ray diffraction pattern, inductively coupled plasma analysis, and bending strength test. In the SEM images, the micro-porous structure of heat-treated bone was changed to sintered ceramic-like structure. The structure of bone mineral from test and control materials was analyzed as100% hydroxyapatite. The ratio of calcium (Ca) to potassium (P), the main inorganic elements, was same even though the Ca and P percentages of the control material was relatively higher than the test material. No death or critical symptoms arose from implantation of the test (chemical treated bone) and control (physiological saline) materials during 12 weeks. The implanted sites were macroscopically examined, with all the groups showing non-irritant results.

Perspectives

Dr. Kwang il Lee
The Scripps Research Institute

Our results indicate that chemical processed bovine bone has a better mechanical property than the heat treated bone and the implantation of this material does not produce systemic or pathological toxicity.

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http://dx.doi.org/10.1016/j.yrtph.2015.10.027

The following have contributed to this page: Dr. Kwang il Lee