What is it about?
Over the past decades, several studies have examined the subcellular localization of the cauliflower mosaic virus (CaMV) P6 protein by tagging it with GFP (P6-GFP). These investigations have been essential in the development of models for inclusion body formation, nuclear transport, and microfilament-associated intracellular movement of P6 inclusion bodies for delivery of virions to plasmodesmata. Although it was shown early on that the translational transactivation function of P6-GFP was comparable to wild type P6, it has not been possible to incorporate a P6-GFP gene into an infectious clone of CaMV. Consequently, it has not been possible to formally prove that a P6-GFP fusion is comparable in function to the unmodified P6 protein.
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Why is it important?
Here we show that transient expression of P6-GFP can complement a defective CaMV replicon through gene expression, replication and encapsidation, which validates the relevance of P6-GFP subcellular localization studies for understanding the development of CaMV infections.
Perspectives
CaMV P6-GFP fusions have been integral for elucidating the subcellular location and function of the multifunctional P6 protein. We demonstrate in the paper that P6-GFP of CaMV can be separately delivered and expressed from the rest of the virus genome, and that this genome arrangement supports the CaMV infection process through replication and virion formation in agroinfiltrated tissues.
Dr. Mustafa Adhab
University of Missouri Columbia
Read the Original
This page is a summary of: Transient expression of cauliflower mosaic virus (CaMV) P6-GFP complements a defective CaMV replicon to facilitate viral gene expression, replication and virion formation, Virology, October 2023, Elsevier,
DOI: 10.1016/j.virol.2023.109854.
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