What is it about?
Circulating antiphospholipid antibodies induce vascular injury and endothelial dysfunction, which are associated with thrombotic events and/or fetal loss. We developed a model in which calibrated automated thrombin generation is carried out in wells lined with cultured endothelial cells. Then we investigated how far b2GP1 antibodies provoked thrombin generation enhancing effects in these cells and/or in blood platelets.
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Why is it important?
Development of thrombin generation assay in endothelial cells to directly evaluate antiphospholipid antibodies pathogenicity.
Perspectives
In this study, we demonstrate the feasibility of using thrombin generation test in cultured endothelial cells. This method allows the assessment of the procoagulant phenotype of interaction of anti-β2GP1 antibodies with platelet and endothelial cells. We suggest that thrombin generation test could be used in cultured cells with the objective of improving therapeutic approaches for patients with APS.
Paul Billoir
Rouen University Hospital Vascular Hemostasis Unit
Read the Original
This page is a summary of: Development of a thrombin generation test in cultured endothelial cells: Evaluation of the prothrombotic effects of antiphospholipid antibodies, Thrombosis Research, September 2018, Elsevier,
DOI: 10.1016/j.thromres.2018.07.021.
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