The best protocol to cryopreserve mouse testicular cells

What is it about?

We studied the effect of different cryopreservation rates, the addition of sugars, different vessels and the addition of an apoptotic inhibitor on the efficiency of testicular cell suspension cryopreservation. After thawing and warming, testicular cell suspensions were transplanted to recipient mice for further functional assay. After selecting the optimal cryopreservation procedure, a second experiment compared the transplantation efficiency between the selected freezing protocol and fresh testicular cell suspensions

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Why is it important?

From a clinical perspective, it was of dire need to study the parameters that grant optimal cryopreservation of mouse testicular cell suspensions.

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