What is it about?
Determination of the fermentation kinetics is one of the main challenges in solid state fermentation. The quantitative measurement of biomass growth during microbial pretreatment by FTIR spectroscopy in Attenuated Total Reflectance mode was evaluated. Samples with different microbial biomass fractions were obtained from two different experiments, i.e., shake flask and fixed-bed reactor experiments. The glucosamine concentration was compared to the normalized absorbance ratio of the 1651 cm−1 to 1593 cm−1 peak, measured by FTIR-ATR.
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Why is it important?
Application of FTIR in ATR mode to follow-up kinetics during solid state fermentation seems to be a fast and easy alternative to laborious measurement techniques, such as glucosamine determination.
Perspectives
The FTIR measurement in ATR mode of solid state fermentation samples showed to provide an intuitive and accurate quantitative method for determination of the growth kinetics that can be easily used by occasional users of the equipment. Future studies will be performed to improve this method and to validate it for other solid state fermentations.
Prof. dr. ir. Iris Cornet
Universiteit Antwerpen
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This page is a summary of: FTIR as an easy and fast analytical approach to follow up microbial growth during fungal pretreatment of poplar wood with Phanerochaete chrysosporium, Journal of Microbiological Methods, February 2018, Elsevier,
DOI: 10.1016/j.mimet.2018.01.004.
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