Long term primary neuron culture a model to study synaptogenesis

What is it about?

In this method we have seen how neuronal growth cone navigate and target to a neuron present at a distance. Also we have observed neurite growth retraction, interstitial axonal branching, pre and post synaptic characterisation and fasciculation. Vesicle dynamics and their glittering behaviour were also seen using viral mediated transduction. Our culture remain healthy for more than 3 month.

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Why is it important?

Simple and easy model to recapitulate the dynamic neural events like neurite growth, growth cone navigation and synaptogenesis. Synapse formation involves coordinated morphometric changes in neural cells. This involves extension of neural projection from cell soma and targeting to a precise neurons presents at a cell distance. In this protocol we have shown how cell after plating shows different morphometric variation and ultimately formation of neural synapses. This paper revolves around growth cone advance, retraction, fasciculation, interstitial branching, pre and post synaptic terminal emergence and ultimately glittering of dynamic vesicles in transfected neurons.

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