A statistical method to quantitate T-cell receptor diversity using one hundred thousand T-cells.

What is it about?

Developed a sensitive assessment of global TCR Vβ repertoire diversity that could be applied in settings of limited T cell numbers, such as in lymphopenic patients or in sorted subsets of T cells.

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Why is it important?

This publication presents a method to quantitate T-cell receptor diversity based hematopoiesis in patients that received adult stem cell transplant treatment for hematologic malignancies. Mayo clinic has started using this method and offering assay to their patients for assessment of T-cell receptor diversity in various clinical contexts including primary immunodeficiencies, monitoring immune reconstitution post hematopoietic cell transplantation, and temporal assessment of repertoire changes in autoimmune diseases and viral infections, using the method published in this paper “Quantitative analysis of T cell receptor diversity in clinical samples of human peripheral blood”.http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/62930 This publication demonstrate that one hundred thousand T cells are sufficient to obtain a robust, highly re-producible measure of the global TCR Vβ repertoire diversity among twenty Vβ families in human peripheral blood. http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/62930

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