Transfection efficiency and transgene expression kinetics of mRNA delivered in naked and nanoparticle format

Kyle K.L. Phua, Kam W. Leong, Smita K. Nair
  • Journal of Controlled Release, March 2013, Elsevier
  • DOI: 10.1016/j.jconrel.2012.12.029

In vivo mRNA nanoparticle delivery; transfection efficiency & kinetics @ 4 different injection sites

What is it about?

Comparing transfection efficiency and kinetics of naked mRNA and mRNA nanoparticles in an animal model. Comparing transfection efficiency and kinetics of naked mRNA and mRNA nanoparticles in an animal model. Comparing transfection efficiency and kinetics of naked mRNA and mRNA nanoparticles in an animal model. Comparing transfection efficiency and kinetics of naked mRNA and mRNA nanoparticles in an animal model. Comparing transfection efficiency and kinetics of naked mRNA and mRNA nanoparticles.

Why is it important?

You will learn: Kinetics are consistent REGARDLESS OF SITE OF INJECTION/ FORMAT OF DELIVERY. SubQ delivery of naked mRNA is more efficient than mRNA NPS. SubQ delivery of naked mRNA last more than 6 days. SubQ delivery of mRNA NPS intranasally last for up to 24 hours.

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http://dx.doi.org/10.1016/j.jconrel.2012.12.029

The following have contributed to this page: Kam Leong and Kyle Phua