Applying DNA affinity chromatography to specifically screen for sucrose-related DNA-binding transcriptional regulators of Xanthomonas campestris

  • Lennart Leßmeier, Rabeaa S. Alkhateeb, Fabian Schulte, Tim Steffens, Tobias Pascal Loka, Alfred Pühler, Karsten Niehaus, Frank-Jörg Vorhölter
  • Journal of Biotechnology, August 2016, Elsevier
  • DOI: 10.1016/j.jbiotec.2016.04.007

Screening for Xanthomonas transcriptional regulators by DNA affinity chromatography

What is it about?

DNA affinity chromatography has been described for the first time as a technique suitable to screen for proteins involved in the regulation of genes in bacteria of the genus Xanthomonas.

Why is it important?

Xanthomonads are important plant pathogens that affect many crops of great agricultural relevance. At the same time they have a role in biotechnology as producers of the polysaccharide xanthan. Regulation of many of their genes is still obscure. DNA affinity chromatography appears as a promising method to reveal new regulatory processes.

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The following have contributed to this page: Dr Frank Jörg Vorhölter