An electrochemical genosensor for Salmonella typhi on gold nanoparticles-mercaptosilane modified screen printed electrode

Ritu Das; Mukesh K. Sharma; Vepa K. Rao; B.K. Bhattacharya; Iti Garg; V. Venkatesh; Sanjay Upadhyay

doi:10.1016/j.jbiotec.2014.08.002

What is it about?

A facile detection of Vi gene of Salmonella typhi using DNA probe, methylene blue is used as an electrochemical indicator which helps in determination of electrochemical signal by producing reduction signal on DNA immobilisation and its hybridisation.

Why is it important?

The DNA biosensors shows excellent performance with high sensitivity and good selectivity. The current response was linear with the target sequence concentrations ranging from 1.0* 10-11 to 0.5 * 10-8M and the detection limit was found to be 50pM

This page is a summary of: An electrochemical genosensor for Salmonella typhi on gold nanoparticles-mercaptosilane modified screen printed electrode, Journal of Biotechnology, October 2014, Elsevier,
DOI: 10.1016/j.jbiotec.2014.08.002.
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An electrochemical genosensor for Salmonella typhi on gold nanoparticles-mercaptosilane modified screen printed electrode
In this work, we fabricated a system of integrated self-assembled layer of organosilane 3- mercaptopropyltrimethoxy silane (MPTS) on the screen printed electrode (SPE) and electrochemically deposited gold nanoparticle for Salmonella typhi detection employing Vi gene as a molecular marker.

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Miss RITU DAS
DEFENCE RESEARCH & DEVELOPMENT ESTABLISHMENT

Electrochemical DNA biosensor for Salmonella typhi

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