CRISPR screens for human genes that mediate susceptibility to Vibrio parahaemolyticus
What is it about?
Many bacterial pathogens use specialized syringes to inject toxic proteins to eukaryotic cells (known as “Type III Secretions Systems” or T3SS). In this work, we harnessed the power of the gene editing technology known as CRISPR to identify the human genes that mediate our susceptibility to the 2 different bacterial syringes of Vibrio parahaemolyticus, the leading cause of seafood-borne gastroenteritis in the world. We found that sulfation and fucosylation of the cell surface promote the interaction of these syringes with human cells.
Why is it important?
These findings reveal the importance of ubiquitous surface modifications for T3SS function, potentially explaining the broad tropism of V. parahaemolyticus, and highlight the utility of genome-wide CRISPR/Cas9 screens to discover processes underlying host-pathogen interactions.
The following have contributed to this page: Dr Carlos J Blondel