Fluorescent mannosides serve as acceptor substrates for glycosyltransferase and sugar-1-phosphate transferase activities in Euglena gracilis membranes

What is it about?

•Assays for the analysis of carbohydrate-active enzymes that rely upon fluorescent acceptor substrates are set out. •New assays are validated by benchmarking against radiochemical work with known glycosyltransferase activities. •The installation of a fluorophore on acceptor substrates was easily achieved through click chemistry. •Fluorescence assays are used to discover GTs activities in Euglena gracilis microsomal membranes.

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