What is it about?
Secondary structure investigation of streptokinase after its solubilization and recovery from E. coli inclusion bodies using various chemicals.
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Why is it important?
Our findings show that the secondary structure distribution of the recombinant protein, obtained following different chemical solubilization systems, was varied. With reducing the concentration of chemicals during IB solubilization, the content of turns plus random coils was diminished and simultaneously the amounts of α- and β-sheets were increased. These kinds of alterations in the secondary structures, occurred following IBs solubilization by low concentration of chemicals, could be related to enhancement in recombinant streptokinase biological activity.
Perspectives
The findings of this article demonstrate that the use of various concentrations of chemicals (singly or in combination) for solubilization of IBs could lead to dissimilar structural conformations in the final recovered target protein. This proves that extreme care must be taken when using IB solubilization and refold pathways so that the refolded recombinant target protein reaches the highest biological potency.
Dr Reza Jalalirad
Pasteur Institute of Iran
Read the Original
This page is a summary of: Structural characterization of recombinant streptokinase following recovery from inclusion bodies using different chemical solubilization treatments, Biotechnology Reports, September 2018, Elsevier,
DOI: 10.1016/j.btre.2018.e00259.
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