Short circuiting a sulfite oxidising enzyme with direct electrochemistry: Active site substitutions and their effect on catalysis and electron transfer

Trevor D. Rapson, Ulrike Kappler, Graeme R. Hanson, Paul V. Bernhardt
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, January 2011, Elsevier
  • DOI: 10.1016/j.bbabio.2010.09.005

Protein film voltametry of sulfite dehydrogenase variants

What is it about?

Study the effect of active site substitutions in sulfite dehydrogenase

Why is it important?

Provides an explanation of for non-sigmoidal voltammetric responses

Perspectives

Trevor Rapson
CSIRO

This is the key paper from my PhD. I was seeing odd shaped voltammograms similar to that observed by Prof Fraser Armstrong. We were able to show that the unexpected results were due to changes in the pathway of electron transfer from the catalytic center to the electrode.

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http://dx.doi.org/10.1016/j.bbabio.2010.09.005

The following have contributed to this page: Trevor Rapson and Ulrike Kappler