What is it about?
We describe here the construction of two cloning vectors for Gram-positive bacteria. One has a large broad range. We demonstrate that the vectors enable stable cloning of multiple fragments in a single step generating insert up to 40 kb.
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Why is it important?
These vectors can be used for multiplex cloning, helping the construction of large synthetic chromosomes in Gram-positive bacteria .
Read the Original
This page is a summary of: Structurally stable Bacillus subtilis cloning vectors, Gene, March 1990, Elsevier,
DOI: 10.1016/0378-1119(90)90495-d.
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