What is it about?
The instability of Paramecium aurelia surface components has been shown: after 60 min incubation of dense cell suspension [(1-2 x 10(6) cells per ml] in Tris-Ca buffer at 4 degrees C or 23 degrees C the surface coat was partially stripped off and the proteins were released to the medium.
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Why is it important?
The electrophoretic analysis of the released proteins shows one major band of mol. wt. 280,000--300,000 and some minor bands. The major released protein is the immobilization antigen as proved using immunodiffusion test and antibody precipitation technique.
Perspectives
Release of proteins is not due to the cell death - viability of cilates was checked by Trypan Blue staining.
Professor Elzbieta Wyroba
Nencki Institute of Experimental Biology
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This page is a summary of: Release of Paramecium immobilization antigen to the non-nutrient medium, Cell Biology International Reports, January 1980, Wiley,
DOI: 10.1016/0309-1651(80)90002-8.
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