Homology-based mutagenesis of an amino acid dehydrogenase

What is it about?

Sequence comparisons established the relatedness of several amino acid dehydrogenases. The existence of a solved structure for GluDH made it possible to interpret key differences by homology modelling. In this paper we used predictions that two residues were primarily responsible for specificity difference between LeuDH and PheDH. Single and double mutations validated the prediction

Featured Image