Evidence that Calu-3 cells are largely resistant to Ebola virus entry
What is it about?
The highly pathogenic Ebola virus (EBOV) can enter into virtually all target cells except lymphocytes and several cellular factors required for entry are known. However, it is largely unknown whether low endogenous expression of these factors can limit viral entry into certain cells. This study shows that the lung derived cell line Calu-3 is largely resistant to entry driven by the glycoprotein of EBOV and several other filoviruses. Entry could be restored upon directed expression of the cysteine protease cathepsin L, which primes the viral glycoprotein for entry and is known to be expressed at low levels in Calu-3 cells, or of the C-type lectin DC-SIGN, which is know to boost EBOV entry into cells.
Why is it important?
The study demonstrates that EBOV glycoprotein-driven entry into target cells can be limited at the stages of attachment and glycoprotein priming.
The following have contributed to this page: Professor Stefan Pöhlmann