What is it about?
Poly (styrene-co-maleic acid) salts (pSMA-S), represent a class of substances known as polymeric surfactants which have been used as emulsifying and dispersing agents for at least half a century. Just a decade ago researchers started to utilize these polymers for extraction of membrane proteins. This approach provides a new opportunity to isolate and characterize multi-subunit membrane protein complexes in their native form and lipid environment. In this paper we systematically characterized the efficacy of tvelve SMA copolymers to extract membrane protein complexes from spinach chloroplast thylakoids.
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Why is it important?
Since the way scientists determine solubilization efficacy varies between different laboratories, it was crucial for a wide variety of pSMA-S to be tested in one laboratory to provide an accurate and direct comparison. Additionally, identifying the SMA copolymer properties responsible for their high solubilization efficacy will allow to further advance the polymer surfactant technology and membrane protein biology.
Read the Original
This page is a summary of: Evaluation of commercially available styrene-co-maleic acid polymers for the extraction of membrane proteins from spinach chloroplast thylakoids, European Polymer Journal, May 2019, Elsevier, DOI: 10.1016/j.eurpolymj.2018.10.035.
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