The effect of thiolated phospholipids on formation of supported lipid bilayers on gold substrates investigated by surface-sensitive methods

  • Abdulhalim Kılıç, M. Fazeli Jadidi, Hakan Özgür Özer, Fatma Neşe Kök
  • Colloids and Surfaces B Biointerfaces, December 2017, Elsevier
  • DOI: 10.1016/j.colsurfb.2017.09.016

The effect of thiolated phospholipids on formation of SLB on gold substrates

What is it about?

A series of unilamellar liposomes with different thiol percentage (0.1 to 100%) was employed to investigate the effect of thiol-terminated phospholipid presence on liposomal behavior and on the effectiveness in SLB formation. The assessment of the quality of the constructed SLB platform was mainly interpreted via QCM-D.

Why is it important?

Most of the model lipid membrane studies on gold involve the usage of various surface-modification strategies to rupture liposomes and induce lipid bilayer formation since liposomes with polar surfaces do not interact with bare, hydrophobic gold. The construction of SLB on gold in a single step has been successfully accomplished.


Dr Abdulhalim KILIÇ
Molecular Biology-Biotechnology and Genetics Research Center

The binding kinetics of thiolated liposomes monitored by QCM-D and SPR methods. Viscoelastic modelling was implemented for the optimized thiol ratio. Spatial surface characterization studies were performed by AFM. Thiolated lipid presence helps SLB formation without prior surface modification. Effect of thiol presence to liposome rupture is reduced for larger liposomes.

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The following have contributed to this page: Dr Abdulhalim KILIÇ